We integrate cryopreservation with data storage in a co-storage box, the Cryobox. Our data storage medium is a unique high resolution photosensitive film (piqlFilm), where we store a combination of human readable and machine readable information.
The CryoBox has been developed through broad and deep research involving materials technology companies, biotech research institutions and genebanks.
The box consists of three main parts:
(1) A lid with permanent numbering to keep track of the position of the sample vials (tubes) for the genetic material.
(2) An inner grid that has space for 72 vials for genetic material, as well as space for keeping the piqlFilm in a dedicated DataTube.
(3) A base that holds the grid and that fits together with the lid.
The CryoBox is made for easy handlingin a lab environment and so that the lid cannot be put on incorrectly. Several laboratories and research institutions have been involved in the design and testing of the CryoBox, such as KU Leuven in Belgium, Luke in Finland, IPK in Germany and the Norwegian Institute for Bioeconomy NIBIO and Sagaplant in Norway. The choice of materials has been important for the CryoBox to withstand the stresses of cryopreservation over time, and together with the Norwegian polymer technology institute, Norner, it has been long-term stress tested, both with respect to the materials selected as well as the functionality and use of the CryoBox. Materials have been carefully selected and tested to ensure nothing leaks out of the materials over time that will affect neither the genetic material nor the data.
CIP has implemented the use of microfilm equipped cryoboxes from GenEver for the international safety copy of its potato cryo-collection (> 4500 accessions)
Rainer VollmerEcuador Country Representative/Scientist,
Second generation CryoBox™
Width, height, depth
Weight. (Dry)
By preserving biological samples and their associated data in a single system, GeneEver's CryoBox eliminates separate archives and ensures the permanent integrity of the material-data link.
GeneEver’s data preservation solution supports the use of the cryo-method, preserving genetic material, e.g. living plant material, in liquid nitrogen at -196° Celsius (-320 Fahrenheit), safeguarding genetic information and plant health status.
GeneEver utilizes Piql’s unique data preservation technology, a secure photosensitive long-term preservation medium, piqlFilm, to store the data related to the genetic material. Data on piqlFilm can be co-stored with the genetic material, or data can be stored separately, in our secure vault.
Another unique product is the DataStrip that can be used for co-storage with e.g. seeds placed in a gene bank or for co-storage with other genetic material that is not stored in cryo.
GeneEver provides a unified, secure, and scalable solution for the co-storage of any biological material—from advancing human health to preserving global biodiversity.
Preserve the future of agriculture and biodiversity. Catalog and store seeds, living plant specimens, and pollen with complete integrity.
Sperm, eggs, embryos, blood, stem cells, tissue, organs, tumours, genetic material for gene therapy etc.
Reproductive materials, cellular materials, tissue, DNA, milk, serum
Virus (e.g. for use in vaccine research and development), bacteria, fungi, and microorganisms
The genebank of the International Potato Center in Peru (CIP) holds the FAO in-trust collection of potato, sweetpotato and other nine Andean root and tuber crops. Although about 90% of the potato collection is already successfully cryobanked at CIP (more than 4500 accessions). GeneEver has entered into an agreement with CIP for the supply of cryoboxes, film and data handling for the safe co-storage of cryopreserved material and data.
IPK Gatersleben Leibniz Institute of Plant Genetics and Crop Plant Research in Germany is the world's 7th largest seed and plant laboratory, and the largest in Europe. In addition, IPK is a significant player in the cryopreservation of potatoes, among other things, with over 2,000 accessions.
GeneEver has entered into an agreement with IPK for the supply of cryoboxes, film and GeneEver Connect for backup copies of IPK's cryopreserved material with associated information.
It is important to share our knowledge, which can be easily forgotten, so that others can benefit from it.